Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Alpha-glucosidase inhibitory and antioxidant activities of various extracts of aerial parts of Fagonia indica Burm. F

Atiq-ur-Rehman ^1,2 , Abida Latif^1,2, Nasir Abbas¹, Imran Waheed³, Atta-ur-Rehman ⁴, Muhammad Naeem Qaisar⁵

¹University College of Pharmacy, University of the Punjab, Lahore, Pakistan; ²Faculty of Pharmacy, The University of Lahore; ³Akhtar Saeed College of Pharmaceutical Sciences, Bahria Town; ⁴Department of Pharmacy, Forman Christian College, Lahore; ⁵College of Pharmacy, University of Sargodha, Sargodha, Pakistan.

For correspondence:- Atiq-ur-Rehman Email: atiqurrehman10@gmail.com

Accepted: 8 March 2019 Published: 30 April 2019

Citation: A, Latif A, Abbas N, Waheed I, A, Qaisar MN. Alpha-glucosidase inhibitory and antioxidant activities of various extracts of aerial parts of Fagonia indica Burm. F. Trop J Pharm Res 2019; 18(4):791-797 doi: 10.4314/tjpr.v18i4.16

© 2019 The authors.
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Abstract

Purpose: To investigate in vitro antioxidant and anti-diabetic potentials of Fagonia indica Burm.f. Var. indica in order to provide scientific evidence for its traditional use.

Methods: Cold maceration method was employed for the preparation of extracts of aerial parts of the plant using chloroform, n-hexane, methanol and water as solvents. Total flavonoid and polyphenolic content of various extracts were determined by standard methods. The antioxidant and anti-diabetic activities of the extracts were determined using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and in vitro α-glucosidase (maltase) inhibitory assays, respectively.

Results: Preliminary results indicated the presence of various phytochemicals in the extracts. The chloroform extract exhibited the highest contents of total flavonoids (65.98 ± 1.63 mg QE/g) and polyphenols (26.75 ± 1.09 mg GAE/g). This extract also showed the highest free radical scavenging (64.74 ± 1.43 %) with IC₅₀ value of 34.18 ± 5.57 µg/mL while the methanol extract exerted the highest α-glucosidase (maltase) inhibitory activity (45.22 ± 0.46 %) with half-maximal concentration (IC₅₀) of 220.4 ± 0.41 µg/mL.

Conclusion: The extracts of the aerial parts of Fagonia indica possess significant anti-diabetic and antioxidant effect, thus justifying the traditional use of the plant for treatment of diabetes.

Keywords: α-Glucosidase, Antioxidant, Fagonia indica, Flavonoids, Polyphenols